ABSTRACT
Objective To develop a sensitive,quick and convenient comet assay with Viciafaba root cells,so as to provide the reference for biomonitoring in situ.Methods The K_2CrO_7 and H_2O_2 were used as the positive control,the Viciafaba root cells isolated with mechanical and enzyme treatment respectively were exposed to the different concentrations of K_2CrO_7(1 nmol/L,10 nmol/L,100 nmol/L,1000 nmol/L)and H202(25 ? mol/L,50 ? mol/L,100 ?mol/L,200 ?mol/L).The effect of DNA damage was detected by using comet assay.Results The yield of Viciafaba root cells isolated from enzyme treatment were much higher than that from mechanical treatment.K_2CrO_7and H_2O_2 could cause DNA damage in Viciafaba root cells,there was significant difference between exposure group and control group(P
ABSTRACT
Objective To have a knowledge of the mutagenicity of contaminates in drinking water in a certain city. Methods Mutagenic activity of organic extracts of source water chloridized water in water plant and tap water from the city were detected with single cell gel-electrophoresis mouse primary hepatocytes in culture were used and Ames test. Results For Ames test the organic extract in 3 liters chloridized water in water plant and tap water samples produced positive result and that in 6 liters source water sample still gave a negative result. For single cell gel-electrophoresis test the organic extract in 0.1 liter chloridized water in water plant tap water and 0.5 liter source water produced positive result. Conclusion The sensitivity of single cell gel-electrophoresis with primary hepatocytes in detection of genotoxicity of cloridized water and source water is much higher than Ames test.